1. Acceptance of These Terms
By accessing or using TM Calculator, you agree to follow these Terms and Conditions. If you do not agree with these terms, please stop using the website.
2. About TM Calculator
TM Calculator provides free online primer melting temperature calculations, primer-related outputs, and educational information for molecular biology and PCR workflows.
The website is intended for informational, educational, and research-support purposes only. It does not replace professional laboratory judgment, validated protocols, manufacturer instructions, or institutional guidelines.
3. Use of the Website
You agree to use this website only for lawful and appropriate purposes. You must not:
- Attempt to damage, overload, hack, or disrupt the website.
- Use automated systems in a way that harms performance or availability.
- Copy, scrape, or republish large portions of the website without permission.
- Use the website for misleading, unlawful, abusive, or harmful activity.
4. Calculator Accuracy and Scientific Disclaimer
We aim to provide useful and accurate calculations based on commonly used thermodynamic methods and PCR assumptions. However, melting temperature and annealing temperature predictions can vary depending on sequence, buffer composition, polymerase system, salts, magnesium, additives, template complexity, and experimental setup.
Results should be treated as guidance, not guaranteed experimental outcomes. Always validate important PCR conditions experimentally, such as through gradient PCR or manufacturer-recommended protocols.
5. No Professional or Medical Advice
TM Calculator does not provide medical, diagnostic, clinical, regulatory, legal, or professional advice. Any scientific information on this website is provided for general educational purposes.
6. User Inputs and Responsibility
You are responsible for the sequences, values, and settings you enter into the calculator. You should verify all outputs before using them in experiments, publications, diagnostics, manufacturing, or other important workflows.
Do not submit sensitive, confidential, proprietary, patient-related, or regulated information through the website.
7. Intellectual Property
The website design, text, layout, code, branding, and original content are owned by TM Calculator unless otherwise stated. You may use the website for personal, academic, and internal research-support purposes, but you may not reproduce or redistribute substantial parts of the website without permission.
8. Third-Party Links
TM Calculator may include links to scientific references, manufacturer resources, external websites, or other third-party pages. These links are provided for convenience and reference only.
We do not control external websites and are not responsible for their content, accuracy, privacy practices, or availability.
9. Advertising, Cookies, and Google AdSense
TM Calculator may display advertisements, including ads served by third-party advertising partners such as Google AdSense. Advertising partners may use cookies or similar technologies to show ads, measure performance, prevent fraud, and improve ad relevance.
Third-party vendors, including Google, may use cookies to serve ads based on a user’s prior visits to this website or other websites. Users may manage ad personalization and cookie choices through their browser settings or applicable advertising settings provided by third-party vendors.
For more information about how we handle privacy, cookies, and data, please read our Privacy Policy.
10. Limitation of Liability
TM Calculator is provided on an “as is” and “as available” basis. We do not guarantee that the website will always be available, error-free, uninterrupted, secure, or suitable for every laboratory or research use.
To the fullest extent permitted by law, TM Calculator and its owners are not liable for any direct, indirect, incidental, consequential, or special damages resulting from your use of the website, calculator results, or external links.
11. Changes to the Website
We may update, modify, remove, or improve any part of the website at any time, including calculator features, formulas, content, pages, and policies.
12. Changes to These Terms
We may update these Terms and Conditions from time to time. Updated terms will be posted on this page with a new effective date. Continued use of the website means you accept the updated terms.
13. Contact Us
If you have questions about these Terms and Conditions, please contact us through the contact page: Contact TM Calculator.